Purpose: To find out the implementation and influence of Puskesmas Godean I training program on sanitation and Escherichia coli contamination on school-based street food vendors in Godean sub-district. Method: The type of research was observational study with cross sectional design. The research sample was school-based street food vendors in the puskesmas catchment area. The sampling technique used was total sampling. Result: This study found that there were significant differences between vendors who took part in the training and those who did not take part in the training in terms of personal hygiene (p = 0.002; p <0.05) and sanitation of food equipment (p = 0.028; p <0.05). While there was no significant difference in terms of food serving sanitation (p = 0.285; p <0.05), sanitation facilities of vendors (p = 0.154; p <0.05), and Escherichia coli contamination (p = 0, 126 ; p <0.05). Conclusion: The training program on the hygiene of sanitation and Escherichia coli contamination for school-based food vendors in the catchment area was not effective. Training programs for school-based food vendors were effective only on personal hygiene and food equipment sanitation. Keywords: training program; personal hygiene; equipment sanitation; sanitation of food serving; Escherichia coli