Shasifitri, Azahra Aqila Nibras (2026) UJI BANDING HASIL PERTUMBUHAN BAKTERI ENTEROCOCCUS FAECALIS PADA MEDIA BLOOD AGAR PLATE DARAH KUDA DAN DARAH DOMBA. Skripsi thesis, Poltekkes Kemenkes Yogyakarta.
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Abstract
A COMPARATIVE STUDY ON THE GROWTH OF ENTEROCOCCUS FAECALIS BACTERIA ON BLOOD AGAR PLATES PREPARED WITH HORSE BLOOD AND SHEEP BLOOD Azahra Aqila1, Eni Kurniati2, Ratih Hardisari3 1,2,3 Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Yogyakarta Jl. Tatabumi No.3 Banyuraden, Gamping, Sleman email: azahraaqilans@gmail.com, ABSTRACT Backgroud: Enterococcus faecalis is an important opportunistic pathogen that can cause various nosocomial infections, such as bacteremia, urinary tract infections, and endocarditis. E. faecalis is known to exhibit hemolytic activity on blood culture media. These characteristics may be influenced by the type of blood used in Blood Agar Plate (BAP) media. However, information regarding the comparison of E. faecalis growth on horse blood and sheep blood BAP remains limited. Objective: This study aims to compare the growth characteristics of E. faecalis on horse blood and sheep blood BAP media. Methods: This study employed a static group comparison design to compare the growth of E. faecalis on horse blood BAP and sheep blood BAP media. Observations were made regarding growth characteristics and hemolysis zones after a 24-hour incubation period. Data were analyzed using an independent two-sample t-test. Results: The results showed that horse blood BAP medium produced clear hemolysis zones (β-hemolysis) and had a larger average colony diameter compared to sheep blood BAP medium. Conclusion: The type of blood in the BAP medium can influence the growth characteristics of E. faecalis. Keyword: Horse Blood, Bacteri Growth, Enterococcus faecalis. UJI BANDING HASIL PERTUMBUHAN BAKTERI ENTEROCOCCUS FAECALIS PADA MEDIA BLOOD AGAR PLATE DARAH KUDA DAN DARAH DOMBA Azahra Aqila1, Eni Kurniati2, Ratih Hardisari3 1,2,3 Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Yogyakarta Jl. Tatabumi No.3 Banyuraden, Gamping, Sleman email: azahraaqilans@gmail.com, ABSTRAK Latar Belakang: Enterococcus faecalis merupakan bakteri patogen oportunistik penting yang dapat menyebabkan berbagai infeksi nosokominal, seperti bakteremia, infeksi saluran kemih dan endokarditis. E. faecalis diketahui dapat menunjukkan aktivitas hemolitik pada media kultur darah. Karakteristik tersebut dapat dipengaruhi oleh jenis darah yang digunakan pada media Blood Agar Plate (BAP). Namun, informasi mengenai perbandingan pertumbuhan E. faecalis pada BAP darah kuda dan darah domba masih terbatas. Tujuan: Mengetahui perbedaan karakteristik pertumbuhan E. faecalis pada media Blood Agar Plate darah kuda dan darah domba. Metode: Penelitian ini menggunakan desain static group comparison dengan membandingkan pertumbuhan E. faecalis pada media BAP darah kuda dan darah domba. pengamatan dilakukan terhadap karakteristik pertumbuhan dan zona hemolisis setelah masa inkubasi 24 jam. data dianalisis menggunakan uji statistik t dua sampel independent. Hasil: Hasil penelitian menunjukkan bahwa media BAP darah kuda menghasilkan zona hemolisis bening (β-hemolisis) dan rata – rata diameter koloni lebih besar dibandingkan dengan media BAP darah domba. Kesimpulan: Jenis darah pada media BAP dapat memengaruhi karakteristik pertumbuhan E. faecalis. Kata Kunci: Darah Kuda, Pertumbuhan Bakteri, Enterococcus faecalis
| Item Type: | Thesis (Skripsi) |
|---|---|
| Kata Kunci/Keyword Abstrak: | Horse Blood, Bacteri Growth, Enterococcus faecalis. Darah Kuda, Pertumbuhan Bakteri, Enterococcus faecalis. |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Poltekkes Kemenkes Yogyakarta > Jurusan Teknologi Laboratorium Medis > Program Studi Sarjana Terapan Teknologi Laboratorium Medis |
| Depositing User: | Mahasiswa Polkesyogya |
| Date Deposited: | 07 Oct 2026 02:18 |
| Last Modified: | 07 Oct 2026 02:18 |
| URI: | http://eprints.poltekkesjogja.ac.id/id/eprint/23837 |
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